HAIRY MERISTEM with WUSCHEL confines CLAVATA3 expression to the outer apical meristem layers
Creators
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1.
California Institute of Technology
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2.
Purdue University West Lafayette
Abstract
The control of the location and activity of stem cells depends on spatial regulation of gene activities in the stem cell niche. Using computational and experimental approaches, we have tested and found support for a hypothesis for gene interactions that specify the Arabidopsis apical stem cell population. The hypothesis explains how the WUSCHEL gene product, synthesized basally in the meristem, induces CLAVATA3-expressing stem cells in the meristem apex but, paradoxically, not in the basal domain where WUSCHEL itself is expressed. The answer involves the activity of the small family of HAIRY MERISTEM genes, which prevent the activation of CLAVATA3 and which are expressed basally in the shoot meristem.
Additional Information
© 2018 American Association for the Advancement of Science. This is an article distributed under the terms of the Science Journals Default License. Received 26 December 2017; accepted 19 June 2018. We are grateful to H. Yang for sharing the HAM1m DNA construct and to A. Garda from Caltech and C. Layug from Purdue for technical support. The work at Caltech was funded by NIH grant R01 GM104244 and by the Howard Hughes Medical Institute and the Gordon and Betty Moore Foundation (through grant GBMF3406 to E.M.M.). The work at Purdue was funded by a start-up package and support from the Purdue Center for Plant Biology to Y.Z. Author contributions: Y.Z., A.Y., and E.M.M. conceived of the research direction; Y.Z., H.H., T.L., and Y.G. performed experiments; A.Y. performed modeling and simulation; Y.Z., X.L., and E.M.M. commented on the model; X.L. contributed reagents; Y.Z., A.Y., and X.L. analyzed and interpreted data; Y.Z. and E.M.M. supervised the project; Y.Z., A.Y., X.L., and E.M.M. wrote the manuscript; and H.H., T.L., and Y.G. commented on the manuscript. The authors declare that they have no competing interests. Data and materials availability: All data are available in the main text or the supplementary materials.Attached Files
Accepted Version - nihms-977997.pdf
Supplemental Material - MoviesS1-15.zip
Supplemental Material - aar8638-Zhou-SM.pdf
Files
aar8638-Zhou-SM.pdf
Additional details
Identifiers
- PMCID
- PMC6095697
- Eprint ID
- 88506
- DOI
- 10.1126/science.aar8638
- Resolver ID
- CaltechAUTHORS:20180802-135135697
Related works
- Describes
- 10.1126/science.aar8638 (DOI)
Funding
- NIH
- R01 GM104244
- Howard Hughes Medical Institute (HHMI)
- Gordon and Betty Moore Foundation
- GBMF3406
- Purdue University
Dates
- Created
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2018-08-02Created from EPrint's datestamp field
- Updated
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2022-03-09Created from EPrint's last_modified field