Published October 2008
| Version Published
Journal Article
Open
An improved cryogen for plunge freezing
-
1.
California Institute of Technology
Abstract
The use of an alkane mixture that remains liquid at 77 K to freeze specimens has advantages over the use of a pure alkane that is solid at 77 K. It was found that a mixture of methane and ethane did not give a cooling rate adequate to produce vitreous ice, but a mixture of propane and ethane did result in vitreous ice. Furthermore, the latter mixture produced less damage to specimens mounted on a very thin, fragile holey carbon substrate.
Additional Information
© Microscopy Society of America 2008. Received December 20, 2007; accepted June 4, 2008. This work was supported in part by NIH grants R01 AI067548 and P50 GM082545 to G.J.J., a Searle Scholar Award to G.J.J., the Beckman Institute at Caltech, and gifts to Caltech from the Gordon and Betty Moore Foundation and Agouron Institute.Attached Files
Published - TIVmm08.pdf
Files
TIVmm08.pdf
Additional details
Identifiers
- PMCID
- PMC3058946
- Eprint ID
- 13355
- Resolver ID
- CaltechAUTHORS:TIVmm08
Funding
- NIH
- R01 AI067548
- NIH
- P50 GM082545
- Searle Scholar Award
- Caltech Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
Dates
- Created
-
2009-04-23Created from EPrint's datestamp field
- Updated
-
2021-11-08Created from EPrint's last_modified field