A proposal for a coordinated effort for the determination of brainwide neuroanatomical connectivity in model organisms at a mesoscopic scale
Creators
- Bohland, Jason1
- Wu, Caizhi1
- Barbas, Helen2
- Bokil, Hemant1
- Bota, Mihail3
- Breiter, Hans C.4
- Cline, Hollis T.1
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Doyle, John C.5
- Freed, Peter J.6
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Greenspan, Ralph J.7
- Haber, Suzanne N.8
- Hawrylycz, Michael9
- Herrera, Daniel G.10, 11
- Hilgetag, Claus C.12
- Huang, Z. Josh1
- Jones, Allan9
- Jones, Edward G.13
- Karten, Harvey J.14
- Kleinfeld, David14
- Kötter, Rolf15, 16
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Lester, Henry A.5
- Lin, John M.1
- Mensh, Brett D.6
- Mikula, Shawn13
- Panskepp, Jaak17
- Price, Joseph L.18
- Safdieh, Joseph
- Saper, Clifford B.19
- Schiff, Nicholas D.
- Schmahmann, Jeremy D.4
- Stillman, Bruce W.1
- Svoboda, Karel20
- Swanson, Larry W.3
- Toga, Arthur W.21
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Van Essen, David C.18
- Watson, James D.1
- Mitra, Partha P.1
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1.
Cold Spring Harbor Laboratory
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2.
Boston University
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3.
University of Southern California
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4.
Massachusetts General Hospital
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5.
California Institute of Technology
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6.
Columbia University Medical Center
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7.
Neurosciences Institute
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8.
University of Rochester Medical Center
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9.
Allen Institute for Brain Science
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10.
Harvard University
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11.
Brigham and Women's Hospital
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12.
Jacobs University
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13.
University of California, Davis
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14.
University of California, San Diego
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15.
Radboud University Nijmegen Medical Centre
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16.
Radboud University Nijmegen
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17.
Washington State University
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18.
Washington University in St. Louis
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19.
Beth Israel Deaconess Medical Center
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20.
Janelia Research Campus
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21.
University of California, Los Angeles
Abstract
In this era of complete genomes, our knowledge of neuroanatomical circuitry remains surprisingly sparse. Such knowledge is critical, however, for both basic and clinical research into brain function. Here we advocate for a concerted effort to fill this gap, through systematic, experimental mapping of neural circuits at a mesoscopic scale of resolution suitable for comprehensive, brainwide coverage, using injections of tracers or viral vectors. We detail the scientific and medical rationale and briefly review existing knowledge and experimental techniques. We define a set of desiderata, including brainwide coverage; validated and extensible experimental techniques suitable for standardization and automation; centralized, open-access data repository; compatibility with existing resources; and tractability with current informatics technology. We discuss a hypothetical but tractable plan for mouse, additional efforts for the macaque, and technique development for human. We estimate that the mouse connectivity project could be completed within five years with a comparatively modest budget.
Additional Information
© 2009 Bohland et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Published March 27, 2009. This paper is a result of discussions at the 2007 and 2008 Brain Architecture Project Banbury Center Meetings, funded by the W. M. Keck Foundation. The sponsors had no role in the conception or preparation of this manuscript.Attached Files
Published - Bohland2009p4251Plos_Comput_Biol.pdf
Files
Bohland2009p4251Plos_Comput_Biol.pdf
Additional details
Identifiers
- PMCID
- PMC2655718
- Eprint ID
- 14612
- Resolver ID
- CaltechAUTHORS:20090717-142050047
Dates
- Created
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2009-07-24Created from EPrint's datestamp field
- Updated
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2021-11-08Created from EPrint's last_modified field