Published September 17, 2003
| Version public
Journal Article
Screening of protein crystallization conditions on a microfluidic chip using nanoliter-size droplets
Abstract
Protein crystallization is a major bottleneck in determining tertiary protein structures from genomic sequence data. This paper describes a microfluidic system for screening hundreds of protein crystallization conditions using less than 4 nL of protein solution for each crystallization droplet. The droplets are formed by mixing protein, precipitant, and additive stock solutions in variable ratios in a flow of water-immiscible fluids inside microchannels. Each droplet represents a discrete trial testing different conditions. The system has been validated by crystallization of several water-soluble proteins.
Additional Information
Copyright © 2003 American Chemical Society. Published In Issue: September 17, 2003. Received July 9, 2003. This work was supported by the Searle Scholars Program and by the Beckman Young Investigator Program, and was performed at the MRSEC microfluidic facility funded by NSF. We thank Professors Keith Moffat, Phoebe Rice, and Chuan He for valuable suggestions, and we thank H. Song and J. Tice for experimental advice.Additional details
Identifiers
- Eprint ID
- 40893
- DOI
- 10.1021/ja037166v
- Resolver ID
- CaltechAUTHORS:20130821-160735877
Funding
- Searle Scholars Program
- Beckman Young Investigator award
- NSF
Dates
- Created
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2013-08-28Created from EPrint's datestamp field
- Updated
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2021-11-10Created from EPrint's last_modified field