CaltechAUTHORS
Published September 1, 2010 | Version Published
Journal Article Open

The RNA binding protein Larp1 regulates cell division, apoptosis and cell migration

Creators

  • 1. ROR icon Imperial College London

Abstract

The RNA binding protein Larp1 was originally shown to be involved in spermatogenesis, embryogenesis and cell-cycle progression in Drosophila. Our data show that mammalian Larp1 is found in a complex with poly A binding protein and eukaryote initiation factor 4E and is associated with 60S and 80S ribosomal subunits. A reduction in Larp1 expression by siRNA inhibits global protein synthesis rates and results in mitotic arrest and delayed cell migration. Consistent with these data we show that Larp1 protein is present at the leading edge of migrating cells and interacts directly with cytoskeletal components. Taken together, these data suggest a role for Larp1 in facilitating the synthesis of proteins required for cellular remodelling and migration.

Additional Information

© The Author(s) 2010. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Received February 16, 2010; Revised April 1, 2010; Accepted April 7, 2010. Paul Bassett from Stats Consultancy Ltd for statistical advice and analysis. Dr Ernesto Yague and Dr Justin Sturge (both Imperial College) for informative discussions and manuscript advice. FUNDING. Biotechnology and Biological Sciences Research Council (BBSRC) (to K.S. studentship, to A.E.W. Professorial Fellowship); Cancer Research UK (to D.M.G.); Wellbeing of Women (to S.-J.L. Entry Level Scholarship) and Experimental Cancer Medicine Centre (ECMC) (to C.B.). Funding for open access charge: Charitable research fund. Conflict of interest statement. None declared.

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Additional details

Identifiers

PMCID
PMC2938220
Eprint ID
106027
Resolver ID
CaltechAUTHORS:20201013-122118207

Funding

Biotechnology and Biological Sciences Research Council (BBSRC)
Cancer Research UK
Wellbeing of Women
Experimental Cancer Medicine Centre

Dates

Created
2020-10-13
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Updated
2021-11-16
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