CaltechAUTHORS
Published March 27, 2013 | Version Published
Journal Article Open

Axonal Translation of β-Catenin Regulates Synaptic Vesicle Dynamics

Creators

  • 1. ROR icon University of North Carolina at Chapel Hill
  • 2. ROR icon California Institute of Technology
  • 3. ROR icon Max Planck Institute for Brain Research
  • 4. ROR icon Goethe University Frankfurt

Abstract

Many presynaptic transcripts have been observed in axons, yet their role in synapse development remains unknown. Using visually and pharmacologically isolated presynaptic terminals from dissociated rat hippocampal neurons, we found that ribosomes and β-catenin mRNA preferentially localize to recently formed boutons. Locally translated β-catenin accumulates at presynaptic terminals, where it regulates synaptic vesicle release dynamics. Thus, local translation of β-catenin is a newly described mechanism for axons to independently functionalize nerve terminals at great distances from cellular somata.

Additional Information

© 2013 the authors. Received June 21, 2012. Revision received January 14, 2013. Accepted February 7, 2013. Author contributions: A.M.T. designed research; A.M.T., J.W., and H.-C.T. performed research; A.M.T. analyzed data; A.M.T. and E.M.S. wrote the paper. We thank Nicole Calakos (Duke University), Dan Albaugh (University of North Carolina) and Richard Weinberg (University of North Carolina) for helpful discussions and comments on the manuscript. We thank Naomi McArthur (California Institute of Technology) and Graham Mulvany (University of North Carolina) for participation in data analysis. A.M.T. acknowledges support from the Eunice Kennedy Shriver National Institute of Child Health & Human Development (K12HD073945) and the Simons Foundation (SFARI #236390 to A.M.T.). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. A.M.T. is a cofounder of Xona Microfluidics.

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Additional details

Identifiers

PMCID
PMC3651888
Eprint ID
38316
Resolver ID
CaltechAUTHORS:20130507-102304932

Funding

NIH
K12HD073945
Simons Foundation
236390

Dates

Created
2013-05-07
Created from EPrint's datestamp field
Updated
2021-11-09
Created from EPrint's last_modified field