CaltechAUTHORS
Published September 27, 2013 | Version Accepted Version
Journal Article Open

The Sixteenth Iron in the Nitrogenase MoFe Protein

Creators

  • 1. ROR icon California Institute of Technology
  • 2. ROR icon University of Freiburg
  • 3. ROR icon University of Minnesota

Abstract

Another iron in the fire: X-ray anomalous diffraction studies on the nitrogenase MoFe protein show the presence of a mononuclear iron site, designated as Fe16, which was previously identified as either Ca^(2+) or Mg^(2+). The position of the absorption edge indicates that this site is in the oxidation state +2. The high sequence conservation of the residues coordinated to Fe16 emphasizes the potential importance of the site in nitrogenase.

Additional Information

© 2013 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. Received: May 6, 2013. Published online: August 21, 2013. The work was supported by the NIH (grant GM45162 to D.C.R.), NSERC PDF award (to L.M.Z.), and Deutsche Forschungsgemeinschaft (grants Ei-520/7 to O.E., An-676/1 to S.L.A.A., and IRTG 1478). G.M. is a Marie Curie International Outgoing Fellow (European Commission, grant no. 252961). We thank staff at Beamline 12–2, Stanford Synchrotron Radiation Lightsource (SSRL) and staff at HXMA Beamline, Canada Light Source (CLS). SSRL is operated for the DOE and supported by its OBER and by the NIH, NIGMS (P41GM103393) and the NCRR (P41RR001209). CLS is supported by NSERC, NRC, CIHR, and the University of Saskatchewan. We acknowledge the Gordon and Betty Moore Foundation, the Beckman Institute, and the Sanofi-Aventis Bioengineering Research Program at Caltech for their generous support of the Molecular Observatory at Caltech. The assistance of Nathan Dalleska and the resources of the Caltech Environmental Analysis Center are greatly appreciated.

Attached Files

Accepted Version - nihms-534176.pdf

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Additional details

Identifiers

PMCID
PMC3891402
Eprint ID
42229
DOI
10.1002/anie.201303877
Resolver ID
CaltechAUTHORS:20131104-141900919

Funding

NIH
GM45162
Natural Sciences and Engineering Research Council of Canada (NSERC)
Deutsche Forschungsgemeinschaft (DFG)
Ei-520/7
Deutsche Forschungsgemeinschaft (DFG)
An-676/1
Deutsche Forschungsgemeinschaft (DFG)
IRTG 1478
Marie Curie Fellowship
252961
OBER
NIH
P41GM103393
NIH
P41RR001209
National Research Council of Canada
Canadian Institutes of Health Research (CIHR)
University of Saskatchewan
Gordon and Betty Moore Foundation
Caltech Beckman Institute
Caltech Sanofi-Aventis Bioengineering Research Program

Dates

Created
2013-11-04
Created from EPrint's datestamp field
Updated
2021-11-10
Created from EPrint's last_modified field