Published June 27, 2014
| Version public
Book Section - Chapter
Noncontiguous SCHEMA Protein Recombination
Contributors
Abstract
SCHEMA is a method of designing protein recombination libraries that contain a large fraction of functional proteins with a high degree of mutational diversity. In the previous chapter, we illustrated the method for designing libraries by swapping contiguous sequence elements. Here, we introduce the NCR ("noncontiguous recombination") algorithm to identify optimal designs for swapping elements that are contiguous in the 3-D structure but not necessarily in the primary sequence. To exemplify the method, NCR is used to recombine three fungal cellobiohydrolases (CBH1s) to produce a library containing more than 500,000 novel chimeric sequences.
Additional Information
© Springer Science+Business Media New York 2014. The authors acknowledge funding from the Institute for Collaborative Biotechnologies through grant W911NF-09-D-0001 from the US Army Research Office and the National Central University, Taiwan, through a Cooperative Agreement for Energy Research Collaboration. M.A.S. is supported by a Resnick Sustainability Institute fellowship.Additional details
Identifiers
- Eprint ID
- 89037
- Resolver ID
- CaltechAUTHORS:20180822-140726128
Funding
- Army Research Office (ARO)
- W911NF-09-D-0001
- National Central University (Taiwan)
- Resnick Sustainability Institute
Dates
- Created
-
2018-08-22Created from EPrint's datestamp field
- Updated
-
2021-11-16Created from EPrint's last_modified field
Caltech Custom Metadata
- Caltech groups
- Resnick Sustainability Institute
- Series Name
- Methods in Molecular Biology
- Series Volume or Issue Number
- 1179