CaltechAUTHORS
Published March 2, 2009 | Version Accepted Version
Journal Article Open

Fluorescence Detection of a Protein-Bound 2Fe2S Cluster

Creators

  • 1. ROR icon California Institute of Technology
  • 2. ROR icon Rice University

Abstract

A fluorescent biosensor is described for 2Fe2S clusters that is composed of green fluorescent protein (GFP) fused to glutaredoxin 2 (Grx2), as illustrated here. 2Fe2S detection is based on the reduction of GFP fluorescence upon the 2Fe2S-induced dimerization of GFP-Grx2. This assay is sufficiently sensitive to detect submicromolar changes in 2Fe2S levels, thus making it suitable for high-throughput measurements of metallocluster degradation and synthesis reactions.

Additional Information

© 2009 Wiley. Issue online: 24 Feb 2009 Version of Record online: 30 Jan 2009 Manuscript Received: 12 Nov 2008 This work was supported by the Robert A. Welch Foundation (C-1614; to J.J.S.), Arnold and Mabel Beckman Foundation (to C.D.S.), and a National Institutes of Health Biotechnology fellowship (5F32M078901; to K.G.H.).

Attached Files

Accepted Version - nihms149605.pdf

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Additional details

Identifiers

PMCID
PMC3897124
Eprint ID
79343
DOI
10.1002/cbic.200800747
Resolver ID
CaltechAUTHORS:20170725-132756192

Funding

Robert A. Welch Foundation
C-1614
Arnold and Mabel Beckman Foundation
NIH Predoctoral Fellowship
5F32M078901

Dates

Created
2017-07-25
Created from EPrint's datestamp field
Updated
2021-11-15
Created from EPrint's last_modified field