CaltechAUTHORS
Published March 30, 2015 | Version Accepted Version + Supplemental Material
Journal Article Open

High-speed label-free functional photoacoustic microscopy of mouse brain in action

Creators

  • 1. ROR icon Washington University in St. Louis
  • 2. ROR icon Texas A&M University

Abstract

We present fast functional photoacoustic microscopy (PAM) for three-dimensional high-resolution, high-speed imaging of the mouse brain, complementary to other imaging modalities. We implemented a single-wavelength pulse-width-based method with a one-dimensional imaging rate of 100 kHz to image blood oxygenation with capillary-level resolution. We applied PAM to image the vascular morphology, blood oxygenation, blood flow and oxygen metabolism in both resting and stimulated states in the mouse brain.

Additional Information

© 2015 Macmillan Publishers Limited. Received 25 April 2014; Accepted 20 February 2015; Published online 30 March 2015. The authors appreciate J. Ballard's close reading of the manuscript. We thank C. Li, C. Chen, W. Chapman and J. Lee for discussions and technical support. This work was sponsored by US National Institutes of Health (NIH) grants DP1 EB016986 (NIH Director's Pioneer Award), R01 CA186567 (NIH Director's Transformative Research Award), 1S10 RR028864 and R01 CA159959 (to L.V.W.), and US National Science Foundation (NSF) grant CMMI-1131758 (to J.Z.). The bright-field microscopy was performed at the Alafi Neuroimaging Laboratory of the Hope Center for Neurological Disorders, Washington University School of Medicine, which is supported by the NIH Neuroscience Blueprint Center Core grant P30 NS057105. Author Contributions: J.Y., J.Z. and L.V.W. conceived of and designed the study. J.Y., J.-M.Y., K.I.M., L.W. and C.-H.H. constructed the imaging system. J.Y., T.T.W.W. and L.L. performed the experiments and analyzed the data. J.Y., L.W. and L.V.W. wrote the manuscript. L.V.W. supervised the whole study. All authors discussed the conceptual and practical implications of the methods. Competing Financial Interests: L.V.W. has a financial interest in Endra, Inc. and Microphotoacoustics, Inc., which, however, did not support this work. K.I.M. also has a financial interest in Microphotoacoustics, Inc.

Attached Files

Accepted Version - nihms677546.pdf

Supplemental Material - nmeth.3336-S1.pdf

Supplemental Material - nmeth.3336-sv1.mov

Supplemental Material - nmeth.3336-sv2.mov

Supplemental Material - nmeth.3336-sv3.mov

Supplemental Material - nmeth.3336-sv4.mov

Supplemental Material - nmeth.3336-sv5.mov

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Additional details

Identifiers

PMCID
PMC4428901
Eprint ID
67935
DOI
10.1038/nmeth.3336
Resolver ID
CaltechAUTHORS:20160615-093234473

Related works

Describes
10.1038/nmeth.3336 (DOI)

Funding

NIH
DP1 EB016986
NIH
R01 CA186567
NIH
1S10 RR028864
NIH
R01 CA159959
NSF
CMMI-1131758
NIH
P30 NS057105

Dates

Created
2016-06-15
Created from EPrint's datestamp field
Updated
2021-11-11
Created from EPrint's last_modified field