Published June 1, 2015
| Version Published
Journal Article
Open
Dynamic structure and protein expression of the live embryonic heart captured by 2-photon light sheet microscopy and retrospective registration
Creators
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1.
California Institute of Technology
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2.
University of Southern California
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3.
University of California, Santa Barbara
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4.
Idiap Research Institute
Abstract
We present an imaging and image reconstruction pipeline that captures the dynamic three-dimensional beating motion of the live embryonic zebrafish heart at subcellular resolution. Live, intact zebrafish embryos were imaged using 2-photon light sheet microscopy, which offers deep and fast imaging at 70 frames per second, and the individual optical sections were assembled into a full 4D reconstruction of the beating heart using an optimized retrospective image registration algorithm. This imaging and reconstruction platform permitted us to visualize protein expression patterns at endogenous concentrations in zebrafish gene trap lines.
Additional Information
© 2015 Optical Society of America. Received 12 Mar 2015; revised 25 Apr 2015; accepted 1 May 2015; published 11 May 2015. This work was funded by Rosen Center for Bioengineering, Caltech, Center of Excellence in Genomic Science (Caltech), NIH/NHGRI Grant # P50HR004071 and FaceBase, NIH Grant # U01DE020063. We thank Leigh Ann Fletcher, Colleen Paquette and Andrey Andreev for fish care.Attached Files
Published - boe-6-6-2056.pdf
Files
boe-6-6-2056.pdf
Additional details
Identifiers
- PMCID
- PMC4473743
- Eprint ID
- 57687
- Resolver ID
- CaltechAUTHORS:20150520-085804089
Funding
- Donna and Benjamin M. Rosen Bioengineering Center
- NIH
- P50HR004071
- FaceBase
- NIH
- U01DE020063
Dates
- Created
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2015-05-20Created from EPrint's datestamp field
- Updated
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2022-06-06Created from EPrint's last_modified field
Caltech Custom Metadata
- Caltech groups
- Rosen Bioengineering Center