Published April 2009
| Version Supplemental Material + Published
Journal Article
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Non-lethal PCR genotyping of single Drosophila
Abstract
In Drosophila, genetic techniques relying on stochastic chromosomal rearrangements involve the generation and screening of a large number of fly stocks to isolate a few lines of interest. Here, we describe a PCR-based method allowing non-lethal molecular characterization of single flies. Using this procedure, individual candidate recombinant animals can be genotyped and selected one generation earlier than with extant methodology and, importantly, before stocks are established. This advance should significantly facilitate several of the most fundamental and routine techniques in Drosophila genetics.
Additional Information
© 2009 BioTechniques. Received 6 October 2008; accepted 17 December 2008. We thank S.M. Lee and B. Zid for comments on the manuscript. This work was supported by funding from a Lawrence L. and Audrey W. Ferguson Fellowship (to G.B.C.), the National Institutes of Health (NIH; grant no. K99AG030493 to W.W.J. and grant nos. AG16630, AG24366, and DK070154 to S.B.), and the National Science Foundation (grant no. MCB-0418479 to S.B.). This paper is subject to the NIH Public Access Policy. The authors declare no competing interests.Attached Files
Published - Carvalho2009p4287Biotechniques.pdf
Supplemental Material - DNA_isolation_and_am_43523a.pdf
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Carvalho2009p4287Biotechniques.pdf
Additional details
Identifiers
- PMCID
- PMC2793178
- Eprint ID
- 15024
- Resolver ID
- CaltechAUTHORS:20090814-090747457
Funding
- Lawrence L. and Audrey W. Ferguson Fellowship
- NIH
- K99AG030493
- NIH
- AG16630
- NIH
- AG24366
- NIH
- DK070154
- NSF
- MCB-0418479
Dates
- Created
-
2009-08-17Created from EPrint's datestamp field
- Updated
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2021-11-08Created from EPrint's last_modified field