CaltechAUTHORS
Published December 4, 2015 | Version Supplemental Material + Accepted Version
Journal Article Open

Single-cell transcriptomics reveals receptor transformations during olfactory neurogenesis

Creators

  • 1. ROR icon Fred Hutchinson Cancer Research Center
  • 2. ROR icon University of Washington
  • 3. ROR icon University of California, Berkeley

Abstract

The sense of smell allows chemicals to be perceived as diverse scents. We used single neuron RNA-Sequencing (RNA-Seq) to explore developmental mechanisms that shape this ability as nasal olfactory neurons mature in mice. Most mature neurons expressed only one of the roughly 1000 odorant receptor genes (Olfrs) available, and that at high levels. However, many immature neurons expressed low levels of multiple Olfrs. Coexpressed Olfrs localized to overlapping zones of the nasal epithelium, suggesting regional biases, but not to single genomic loci. A single immature neuron could express Olfrs from up to seven different chromosomes. The mature state in which expression of Olfr genes is restricted to one per neuron emerges over a developmental progression that appears independent of neuronal activity requiring sensory transduction molecules.

Additional Information

© 2015 American Association for the Advancement of Science. 13 August 2015; accepted 27 October 2015. We thank J. Delrow, A. Marty, and A. Dawson at the Fred Hutchinson Cancer Research Center (FHCRC) Genomics Facility for assistance with RNA-seq; M. Fitzgibbon and J. Davidson at the FHCRC Bioinformatics Resource for early assistance with sequence analyses; and J. Vasquez and the FHCRC Scientific Imaging Facility for help with confocal microscopy. We also thank members of the Buck laboratory for helpful discussions. This work was supported by the Howard Hughes Medical Institute (L.B.B.), NIH grants R01 DC009324 (L.B.B.) and DP2 HD088158 (C.T.), an Alfred P. Sloan Fellowship (C.T.), and a Dale F. Frey Award for Breakthrough Scientists from the Damon Runyon Cancer Research Foundation (C.T.). L.B.B. is on the Board of Directors of International Flavors & Fragrances. The supplementary materials contain additional data. N.K.H., C.T., and L.B.B. designed the research; N.K.H. and C.T. performed the research; N.K.H., C.T., K.K., Z.L., D.K., X.Y., X.Q., and L.B.B. analyzed the data; L.P. provided guidance; and N.K.H, C.T., and L.B.B. wrote the paper. Raw sequencing data related to this study have been archived in the Gene Expression Omnibus (GEO) database under accession number GSE75413 (available at www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE75413).

Attached Files

Accepted Version - nihms909331.pdf

Supplemental Material - 04/science.aad2456.DC1/aad2456-Hanchate-SM.pdf

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Additional details

Identifiers

PMCID
PMC5642900
Eprint ID
74703
DOI
10.1126/science.aad2456
Resolver ID
CaltechAUTHORS:20170303-130217211

Related works

Describes
http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE75413 (URL)

Funding

Howard Hughes Medical Institute (HHMI)
NIH
R01 DC009324
NIH
DP2 HD088158
Alfred P. Sloan Foundation
Damon Runyon Cancer Research Foundation

Dates

Created
2017-03-03
Created from EPrint's datestamp field
Updated
2021-11-11
Created from EPrint's last_modified field