CaltechAUTHORS
Published February 18, 2011 | Version Published
Book Section - Chapter Open

High speed, inverted optical-resolution photoacoustic microscopy

Creators

  • 1. ROR icon Washington University in St. Louis
  • 2. ROR icon University of Southern California

Contributors

Abstract

Photoacoustic microscopy (PAM) offers label-free, optical absorption contrast. A high-speed, high-resolution PAM system in an inverted microscope configuration with a laser pulse repetition rate of 100,000 Hz and a stationary ultrasonic transducer was built. Four-dimensional in vivo imaging of microcirculation in mouse skin was achieved at 18 three-dimensional volumes per second with repeated two-dimensional raster scans of 100 by 50 points. The corresponding twodimensional B-scan (50 A-lines) frame rate was 1800 Hz, and the one-dimensional A-scan rate was 90,000 Hz. The lateral resolution is 0.23±0.03 μm for Au nano-wire imaging, which is 2.0 times below the diffraction limit.

Additional Information

© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE). We gratefully thank the National Institutes of Health for supporting grants R01 EB000712, R01 EB008085, R01 CA134539, U54 CA136398, R01 CA157277, P41-EB2182. L.W. has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work.

Attached Files

Published - 78990T.pdf

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Additional details

Additional titles

Alternative title
High speed inverted optical-resolution photoacoustic microscopy

Identifiers

Eprint ID
89802
Resolver ID
CaltechAUTHORS:20180920-130934526

Funding

NIH
R01 EB000712
NIH
R01 EB008085
NIH
R01 CA134539
NIH
U54 CA136398
NIH
R01 CA157277
NIH
P41-EB2182

Dates

Created
2018-09-20
Created from EPrint's datestamp field
Updated
2021-11-16
Created from EPrint's last_modified field

Caltech Custom Metadata

Series Name
Proceedings of SPIE
Series Volume or Issue Number
7899