CaltechAUTHORS
Published November 15, 2009 | Version public
Journal Article Metadata-only

Identification of the amino acid-AZT-phosphoramidase by affinity T7 phage display selection

Creators

  • 1. ROR icon University of Minnesota
  • 2. ROR icon Shanghai Public Health Clinical Center

Abstract

A CEM cell cDNA T7 phage display library was prepared and used to screen for activating enzymes of phosphoramidate prodrugs of AZT monophosphate. Although, inefficient compared to ribonucleotide based phosphoramidates, hHint 1 was identified as the likely intracellular pronucleotide activating enzyme.

Additional Information

© 2009 Published by Elsevier. Received 25 July 2009, Revised 17 September 2009, Accepted 17 September 2009, Available online 22 September 2009. This work was partially supported by Grant CA-61909 and the University of Minnesota Graduate School (C.R.W).

Additional details

Identifiers

Eprint ID
114057
DOI
10.1016/j.bmcl.2009.09.067
Resolver ID
CaltechAUTHORS:20220323-565985000

Related works

Describes
10.1016/j.bmcl.2009.09.067 (DOI)

Funding

University of Minnesota

Dates

Created
2022-03-24
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Updated
2022-03-24
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