CaltechAUTHORS
Published December 23, 2013 | Version Supplemental Material + Accepted Version
Journal Article Open

A Chemical Epitope-Targeting Strategy for Protein Capture Agents: The Serine 474 Epitope of the Kinase Akt2

Creators

  • 1. ROR icon California Institute of Technology
  • 2. ROR icon The University of Texas MD Anderson Cancer Center

Abstract

Target and click: Peptide ligands targeted to the C-terminal motif of the kinase Akt2 were obtained by combining phosphate recognition of a dinuclear zinc(II) complex with in situ click chemistry to target this epitope. The peptide ligands (shown as XXXXX) selectively bind the C-terminal polypeptide of Akt2, and are selective for Akt2 relative to the Akt1 and Akt3 isoforms. The ligands differentially modulate Akt2 activity.

Additional Information

© 2013 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. Received: July 7, 2013; Revised: September 23, 2013. Article first published online: 19 Nov. 2013. This work was supported by the Institute for Collaborative Biotechnologies through grant W911NF-09-0001 from the U.S. Army Research Office. Additional personnel or facilities support from the National Cancer Institute (5U54 CA119347: J.R.H. PI), the Grand Duchy of Luxembourg, and the Bill and Melinda Gates Foundation is acknowledged.

Attached Files

Accepted Version - nihms589080.pdf

Supplemental Material - anie_201305882_sm_miscellaneous_information.pdf

Files

anie_201305882_sm_miscellaneous_information.pdf

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Additional details

Identifiers

PMCID
PMC4059071
Eprint ID
42839
Resolver ID
CaltechAUTHORS:20131204-154453202

Funding

Army Research Office (ARO)
W911NF-09-0001
NIH
5U54 CA119347
Grand Duchy of Luxembourg
Bill and Melinda Gates Foundation
National Cancer Institute

Dates

Created
2013-12-05
Created from EPrint's datestamp field
Updated
2021-11-10
Created from EPrint's last_modified field