CaltechAUTHORS
Published February 6, 2012 | Version Supplemental Material + Accepted Version
Journal Article Open

Deep-Tissue Photoacoustic Tomography of a Genetically Encoded Near-Infrared Fluorescent Probe

Creators

  • 1. ROR icon Albert Einstein College of Medicine
  • 2. ROR icon Washington University in St. Louis

Abstract

Skin-deep: The combination of a near-infrared fluorescent protein (iRFP) and deep-tissue photoacoustic tomography clearly demonstrates the superiority of iRFP over other genetically encoded probes. Impressive resolution (280 μm lateral and 75 μm axial) was obtained at a depth of 4 mm in a live animal, and volumetric images of a tumor were produced (see image), thus allowing the spatially resolved monitoring of its development.

Additional Information

© 2012 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. Received: October 5, 2011; Published online: December 23, 2011. We thank Bojana Gligorijevic for help with the mice, and Jeffrey Segall for providing the EGFP-expressing MTLn3 cells. We are grateful to Benjamin Glick (University of Chicago), Dmitry Chudakov and Konstantin Lukyanov (both from the Institute of Bioorganic Chemistry, Russia) for the plasmids encoding GFP-like proteins. We thank Yu Zhang and Yunan Xia for help with maintaining cell cultures. We thank James Ballard for help with editing the manuscript. This work was supported by the National Institutes of Health grants GM073913 and CA164468 (to V.V.V.), and in part by EB000712, EB008085, CA134539, and CA136398 (to L.V.W.).

Attached Files

Accepted Version - nihms-356402.pdf

Supplemental Material - anie_201107026_sm_miscellaneous_information.pdf

Files

anie_201107026_sm_miscellaneous_information.pdf

Files (1.7 MB)

Name Size
md5:e42978c6e01f0770abb9554917a780a7
260.6 kB Preview Download
md5:d8b0c23f032e5eb01924567e7d125276
1.4 MB Preview Download

Additional details

Additional titles

Alternative title
Deep-tissue photoacoustic tomography of genetically encoded iRFP probe

Identifiers

PMCID
PMC3293502
Eprint ID
68869
DOI
10.1002/anie.201107026
Resolver ID
CaltechAUTHORS:20160706-150023634

Related works

Describes
10.1002/anie.201107026 (DOI)

Funding

NIH
GM073913
NIH
CA164468
NIH
EB000712A2S1
NIH
EB008085
NIH
CA134539
NIH
CA136398

Dates

Created
2016-07-07
Created from EPrint's datestamp field
Updated
2021-11-11
Created from EPrint's last_modified field