CaltechAUTHORS
Published October 31, 2018 | Version Published
Journal Article Open

Whole-Brain Analysis of Cells and Circuits by Tissue Clearing and Light-Sheet Microscopy

Creators

  • 1. ROR icon TU Wien
  • 2. ROR icon Medical University of Vienna
  • 3. ROR icon LMU Klinikum
  • 4. ROR icon Ludwig-Maximilians-Universität München
  • 5. ROR icon Munich Cluster for Systems Neurology
  • 6. ROR icon California Institute of Technology
  • 7. ROR icon University of Southern California
  • 8. ROR icon RIKEN
  • 9. ROR icon Massachusetts Institute of Technology
  • 10. ROR icon Broad Institute
  • 11. ROR icon University of Tokyo
  • 12. ROR icon RIKEN Center for Biosystems Dynamics Research

Abstract

In this photo essay, we present a sampling of technologies from laboratories at the forefront of whole-brain clearing and imaging for high-resolution analysis of cell populations and neuronal circuits. The data presented here were provided for the eponymous Mini-Symposium presented at the Society for Neuroscience's 2018 annual meeting.

Additional Information

© 2018 the authors. Beginning six months after publication the Work will be made freely available to the public on SfN's website to copy, distribute, or display under a Creative Commons Attribution 4.0 International (CC BY 4.0) license (https://creativecommons.org/licenses/by/4.0/). Received Aug. 31, 2018; revised Sept. 27, 2018; accepted Sept. 27, 2018. The Dodt laboratory thanks Dr. Saiedeh Saghafi and Dr. Klaus Becker for aspheric optics-based LSFM work, and the Austrian science funding agency FWF and the German Hertie foundation for support; the Ertürk laboratory thanks Ruiyao Cai for uDISCO figure and Synergy Excellence Cluster Munich (SyNergy), Fritz Thyssen Stiftung, and DFG for support; the Miyawaki laboratory thanks Dr. Hiroshi Hama for ScaleS figure and Grant-in-Aid for Scientific Research on Priority Areas (JSPS KAKENHI), the Human Frontier Science Program, and the Brain Mapping by Integrated Neurotechnologies for Disease Studies (Brain/MINDS) for support; the Ueda laboratory thanks Tatsuya Murakami for offering picture materials for CUBIC figure, and Brain/MINDS (AMED/MEXT), the Basic Science and Platform Technology Program for Innovative Biological Medicine (AMED/MEXT)and Grant-in-Aid for Scientific Research (S) (JSPS KAKENHI) for support; the Chung laboratory thanks The Packard Award, the McKnight Foundation and the NIH (1-DP2-ES027992) for support; the Gradinaru laboratory thanks Sripriya Ravindra Kumar, Gerard M. Coughlin, Rosemary Challis, and Collin Challis for CREATE and VAST images, Min Jee Jang for HCR images, and J. Ryan Cho for GCaMP imaging, and the NIH BRAIN Initiative and NIH Director's Office and NSF Neuronex for support. The authors declare no competing financial interests.

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Additional details

Identifiers

PMCID
PMC6706004
Eprint ID
90628
Resolver ID
CaltechAUTHORS:20181105-084438080

Funding

FWF Der Wissenschaftsfonds
Hertie Foundation
Synergy Excellence Cluster
Fritz Thyssen Stiftung
Deutsche Forschungsgemeinschaft (DFG)
Japan Society for the Promotion of Science (JSPS)
Human Frontier Science Program
Brain Mapping by Integrated Neurotechnologies for Disease Studies
Ministry of Education, Culture, Sports, Science and Technology (MEXT)
David and Lucile Packard Foundation
McKnight Foundation
NIH
1-DP2-ES027992
NSF

Dates

Created
2018-11-06
Created from EPrint's datestamp field
Updated
2021-11-16
Created from EPrint's last_modified field