CaltechAUTHORS
Published May 1992 | Version public
Journal Article Metadata-only

Calculation of sequence divergence from the thermal stability of DNA heteroduplexes

Creators

  • 1. ROR icon California Institute of Technology

Abstract

Measurements are reported of the thermal stability of DNA heteroduplexes between clones of the eta-globin pseudogene from a variety of primates. The known sequences of this 7.1-kb region differ from each over a range from 1.6% for human versus chimp to nearly 12% for human versus spider monkey. Thermal stability was determined by standard hydroxyapatite thermal elution, and the results show a precisely linear decrease in thermal stability with divergence. The slope of the regression line is 1.18% sequence divergence per degree centigrade reduction in thermal stability.

Additional Information

© 1992 Springer. Received September 30, 1991 / Accepted December 3, 1991. We thank Jeffrey R. Powell for helpful comments on an earlier draft of this manuscript. M.S. was supported by N.I.H. Training Grant HD-07257. This work was supported by a Developmental Biology Grant from the Lucille P. Markey Charitable Trust.

Additional details

Identifiers

Eprint ID
63058
Resolver ID
CaltechAUTHORS:20151218-081157341

Funding

NIH
HD-07257
Lucille P. Markey Charitable Trust

Dates

Created
2016-01-29
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Updated
2021-11-10
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