Published September 4, 2009
| Version Accepted Version + Supplemental Material
Journal Article
Open
Introduction of an Aliphatic Ketone into Recombinant Proteins in a Bacterial Strain that Overexpresses an Editing-Impaired Leucyl-tRNA Synthetase
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1.
University of California, Los Angeles
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2.
University of Southern California
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3.
California Institute of Technology
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4.
Princeton University
Abstract
Like leucine? A leucine analogue containing a ketone has been incorporated into proteins in E. coli. Only E. coli strains overexpressing an editing-deficient leucyl-tRNA synthetase were capable of synthesizing proteins with the aliphatic ketone amino acid. Modification of ketone-containing proteins under mild conditions has been demonstrated.
Additional Information
Copyright © 2009 WILEY-VCH. Received: July 3, 2009. Published online on August 7, 2009. We thank Dr. Mona Shahgholi for assistance with MALDI-TOF mass spectrometry. This work was supported by NIH Grant R01-GM62523 and by the NSF Center for the Science and Engineering of Materials at Caltech. Y.T. was supported by a fellowship from the Whitaker Foundation, J.A.V. was supported by a National Defense Science and Engineering Graduate Fellowship, and A.J.L. was supported by a National Science Foundation Graduate Research Fellowship. Detailed experimental protocols can be found in the Supporting Information.Attached Files
Accepted Version - nihms327143.pdf
Supplemental Material - sm001.pdf
Files
nihms327143.pdf
Additional details
Identifiers
- PMCID
- PMC3184883
- Eprint ID
- 16247
- Resolver ID
- CaltechAUTHORS:20091012-125839216
Funding
- NIH
- R01-GM62523
- NSF
- Whitaker Foundation
- National Defense Science and Engineering Graduate (NDSEG) Fellowship
Dates
- Created
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2009-10-13Created from EPrint's datestamp field
- Updated
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2021-11-08Created from EPrint's last_modified field