CaltechAUTHORS
Published January 2017 | Version public
Book Section - Chapter Metadata-only

Gas perfusable microfabricated membranes for high-density cell culture

Creators

Abstract

We report for the first time the design, fabrication, and feasibility of a high-density cell culture system capable of a cell substrate density of 40 cm^2/cm^3, greater than 250× current commercially available systems. This design provides oxygen directly from gas perfusable membranes on which the cells are grown to overcome oxygen delivery limitations in traditional culture systems. With translation in mind, the membranes are composed only of medical grade PDMS and Parylene to mitigate contaminant leaching concerns.

Additional Information

© 2017 IEEE. Thanks to the Caltech MEMS Lab members for their guidance with techniques and fabrication. In memory of Lillian Boasie and Gweneth Goodman.

Additional details

Identifiers

Eprint ID
74672
Resolver ID
CaltechAUTHORS:20170302-134637147

Dates

Created
2017-03-03
Created from EPrint's datestamp field
Updated
2021-11-11
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