Published 2012
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Genome-wide profiling of uncapped mRNA
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Abstract
Gene transcripts are under extensive posttranscriptional regulation, including the regulation of their stability. A major route for mRNA degradation produces uncapped mRNAs, which can be generated by decapping enzymes, endonucleases, and small RNAs. Profiling uncapped mRNA molecules is important for the understanding of the transcriptome, whose composition is determined by a balance between mRNA synthesis and degradation. In this chapter, we describe a method to profile these uncapped mRNAs at the genome scale.
Additional Information
© 2012 Springer Science+Business Media, LLC. The authors would like to thank Dr. E.M. Meyerowitz for his encouragement and advice. This work was supported by the US National Science Foundation 2010 Project Grant 0520193, by the National Natural Science Foundation of China Grant 31171159, and by the Millard and Muriel Jacobs Genetics and Genomics Laboratory at the California Institute of Technology.Attached Files
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A9R9216.pdf
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Identifiers
- Eprint ID
- 31571
- Resolver ID
- CaltechAUTHORS:20120521-111710017
Funding
- NSF
- MCB-0520193
- National Natural Science Foundation of China
- 31171159
- Caltech Millard and Muriel Jacobs Genetics and Genomics Laboratory
Dates
- Created
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2012-05-21Created from EPrint's datestamp field
- Updated
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2021-11-09Created from EPrint's last_modified field
Caltech Custom Metadata
- Series Name
- Methods in Molecular Biology
- Series Volume or Issue Number
- 876