Published April 22, 2005
| Version Accepted Version
Journal Article
Open
A Microfluidic Approach for Screening Submicroliter Volumes against Multiple Reagents by Using Preformed Arrays of Nanoliter Plugs in a Three-Phase Liquid/Liquid/Gas Flow
Abstract
Plugging a gap in screening: Arrays of nanoliter-sized plugs of different compositions can be preformed in a three-phase liquid/liquid/gas flow. The arrays can be transported into a microfluidic channel to test against a target (see schematic representation), as demonstrated in protein crystallization and an enzymatic assay.
Additional Information
© 2005 WILEY-VCH. Issue published online: 21 APR 2005. Article first published online: 22 MAR 2005. Manuscript Received: 9 DEC 2004. This work was supported by the National Institutes of Health (grant no. R01 EB001903) and by the Beckman Young Investigator Program and was performed at the MRSEC microfluidic facility funded by the National Science Foundation. We thank David Adamson for invaluable experimental assistance.Attached Files
Accepted Version - nihms14606.pdf
Files
nihms14606.pdf
Additional details
Identifiers
- PMCID
- PMC1766320
- Eprint ID
- 40892
- DOI
- 10.1002/anie.200462857
- Resolver ID
- CaltechAUTHORS:20130821-160735736
Funding
- NIH
- R01 EB001903
- Arnold and Mabel Beckman Foundation
- NSF
Dates
- Created
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2013-08-28Created from EPrint's datestamp field
- Updated
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2023-06-02Created from EPrint's last_modified field