Published March 2011
| Version Supplemental Material
Journal Article
Open
High-throughput single-molecule optofluidic analysis
Creators
-
1.
University of California, Los Angeles
-
2.
Stanford University
-
3.
Howard Hughes Medical Institute
-
4.
California NanoSystems Institute
-
5.
California Institute of Technology
Abstract
We describe a high-throughput, automated single-molecule measurement system, equipped with microfluidics. The microfluidic mixing device has integrated valves and pumps to accurately accomplish titration of biomolecules with picoliter resolution. We demonstrate that the approach enabled rapid sampling of biomolecule conformational landscape and of enzymatic activity, in the form of transcription by Escherichia coli RNA polymerase, as a function of the chemical environment.
Additional Information
© 2011 Nature Publishing Group. Received 09 July 2010; Accepted12 January 2011; Published online 06 February 2011. We thank R. Colyer, P. Blainey and other members of the Weiss and Quake laboratories for helpful discussions. This work was supported by US National Science Foundation Frontiers in Integrative Biological Research grant 0623664 and National Institutes of Health grant GM069709. Fluorescence spectroscopy was performed at the University of California, Los Angeles and California NanoSystems Institute Advanced Light Microscopy and Spectroscopy Shared Facility. A.M.S. was supported by the Stanford University Diversifying Academia, Recruiting Excellence fellowship. Author Contributions: S.K., A.M.S. and D.S.M. designed experiments, conducted experiments, wrote and implemented data acquisition and analysis software, and analyzed data. R.R.L. analyzed data. S.K., A.M.S., S.R.Q., S.W. and D.S.M. assisted in writing and editing of the manuscript.Attached Files
Supplemental Material - nmeth.1569-S1.pdf
Supplemental Material - nmeth.1569-S2.zip
Files
nmeth.1569-S1.pdf
Additional details
Identifiers
- Eprint ID
- 22871
- Resolver ID
- CaltechAUTHORS:20110314-150848330
Funding
- NSF Frontiers in Integrative Biological Research (FIBR)
- 0623664
- NIH
- GM069709
- Stanford University
Dates
- Created
-
2011-03-15Created from EPrint's datestamp field
- Updated
-
2021-11-09Created from EPrint's last_modified field