A wearable percutaneous implant for long term zebrafish epicardial ECG recording

Heart, as the least regenerative organ in human, can regenerate in zebrafish even after up to 20% ventricle amputation without forming any scar tissue. The Electrocardiogram (ECG) recorded from multiple fixed locations around the heart with high spatial resolution can elucidate changes in cardiac electric phenotypes of injured and regenerating tissues. Therefore, a wearable zebrafish harness with a percutaneous planar electrode array was designed, fabricated and properly positioned to uncover the electrical conduction phenotypes during the zebrafish heart regeneration. The recording circuitry and data processing have also been developed and customized, paving the way to the future underwater wireless long-term ECG recording in real time.