Photoacoustic microscopy with an enhanced axial resolution of 5.8 μm
Contributors
Abstract
The axial resolution of photoacoustic microscopy (PAM) can be enhanced by reducing the speed of sound within the imaging region of interest. This principle was demonstrated on a previously-reported PAM system, which utilized a 125 MHz ultrasonic transducer for signal detection and the Wiener deconvolution for signal processing. With sound slowed by silicone oil immersion, we have achieved a finest axial resolution of 5.8 μm for PAM, as validated by phantom experiments. The axial resolution was also enhanced in vivo when mouse ears injected with silicone oil were imaged. After injection of silicone oil, the blood vessels were resolved more clearly. When tissue-compatible low-speed liquids become available, this approach may find applications in PAM as well as in other imaging modalities, such as photoacoustic computed tomography and ultrasound imaging.
Additional Information
© 2014 Society of Photo-Optical Instrumentation Engineers. This work was sponsored in part by National Institutes of Health grants DP1 EB016986 (NIH Director's Pioneer Award), R01 CA134539, U54 CA136398, R01 CA157277, and R01 CA159959. L.W. has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work.Attached Files
Published - 894316.pdf
Files
894316.pdf
Additional details
Identifiers
- Eprint ID
- 89565
- Resolver ID
- CaltechAUTHORS:20180912-095829705
Funding
- NIH
- DP1 EB016986
- NIH
- R01 CA134539
- NIH
- U54 CA136398
- NIH
- R01 CA157277
- NIH
- R01 CA159959
Dates
- Created
-
2018-09-12Created from EPrint's datestamp field
- Updated
-
2021-11-16Created from EPrint's last_modified field
Caltech Custom Metadata
- Series Name
- Proceedings of SPIE
- Series Volume or Issue Number
- 8943