CaltechAUTHORS
Published April 2014 | Version Accepted Version + Supplemental Material
Journal Article Open

Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering

Creators

  • 1. ROR icon Columbia University
  • 2. ROR icon Baylor College of Medicine

Abstract

Sensitive and specific visualization of small biomolecules in living systems is highly challenging. We report stimulated Raman-scattering imaging of alkyne tags as a general strategy for studying a broad spectrum of small biomolecules in live cells and animals. We demonstrate this technique by tracking alkyne-bearing drugs in mouse tissues and visualizing de novo synthesis of DNA, RNA, proteins, phospholipids and triglycerides through metabolic incorporation of alkyne-tagged small precursors.

Additional Information

© 2014 Macmillan Publishers Limited. Received 2 October 2013; accepted 29 January 2014; published online 2 March 2014. We thank L. Zhang, L. Brus, V.W. Cornish, D. Peterka and R. Yuste for helpful discussions. We are grateful to Y. Shin and X. Gao for technical assistance. W.M. acknowledges support from Columbia University, a US National Institutes of Health Director's New Innovator Award, the US Army Research Office (W911NF-12-1-0594) and an Alfred P. Sloan Research Fellowship. Author Contributions: L.W., F.H., Y.S., Z.C., Y.Y., C.-C.L. and M.C.W. performed experiments and analyzed data. L.W. and W.M. conceived the concept, designed the experiments and wrote the paper. Competing interests: Columbia University, which L.W., F.H., Y.S., Z.C. and W.M. are affiliated with, has filed a patent application based on this work.

Attached Files

Accepted Version - nihms-566357.pdf

Supplemental Material - nmeth.2878-S1.pdf

Files

nihms-566357.pdf

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Additional details

Identifiers

PMCID
PMC4040164
Eprint ID
86929
DOI
10.1038/nmeth.2878
Resolver ID
CaltechAUTHORS:20180608-133705699

Related works

Describes
10.1038/nmeth.2878 (DOI)

Funding

Columbia University
NIH
Army Research Office (ARO)
W911NF-12-1-0594
Alfred P. Sloan Foundation

Dates

Created
2018-06-08
Created from EPrint's datestamp field
Updated
2021-11-15
Created from EPrint's last_modified field