Non-coding RNA as a trigger of neuropathologic disorder phenotypes in transgenic Drosophila
Abstract
At most, many protein-misfolding diseases develop as environmentally induced sporadic disorders. Recent studies indicate that the dynamic interplay between a wide repertoire of noncoding RNAs and the environment play an important role in brain development and pathogenesis of brain disorders. To elucidate this new issue, novel animal models which reproduce the most prominent disease manifestations are required. For this, transgenic Drosophila strains were constructed to express small highly structured, non-coding RNA under control of a heat shock promoter. Expression of the RNA induced formation of intracellular aggregates revealed by Thioflafin T in embryonic cell culture and Congo Red in the brain of transgenic flies. Also, this strongly perturbed the brain control of locomotion monitored by the parameters of sound production and memory retention of young 5-day-old males. This novel model demonstrates that expression of non-coding RNA alone is sufficient to trigger neuropathology.
Additional Information
© 2008 Springer. Received: 29 April 2008; Accepted: 1 June 2008; Published online: 9 September 2008. The study was supported by Q-RNA Firm 799 (USA) and Grants of Russian Foundation for Fundamental Research 800 (E.S–P, A.P, M.E, O.Z) and Grant Molecular and Cellular Biology to M.E.External Files
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Additional details
Identifiers
- Eprint ID
- 12752
- Resolver ID
- CaltechAUTHORS:SAVjnt08
Funding
- Q-RNA
- Russian Foundation for Fundamental Research
- 800
Dates
- Created
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2008-12-22Created from EPrint's datestamp field
- Updated
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2021-11-08Created from EPrint's last_modified field