CaltechAUTHORS
Published November 29, 1996 | Version Published
Journal Article Open

Subunit Stoichiometry of a Heteromultimeric G protein-coupled Inward-rectifier K^+ Channel

Creators

  • 1. ROR icon California Institute of Technology

Abstract

We investigated the stoichiometry of the heteromultimeric G protein-coupled inward-recitfier K^+ channel (GIRK) formed from GIRK1 and GIRK4 subunits. Multimeric GIRK constructs with several concatenated channel subunits were expressed in Xenopus oocytes. Coexpression of various trimeric constructs with different monomers clearly showed that the functional channel has stoichiometry (GIRK1)_2(GIRK4)_2. Efforts to establish a preferred arrangement of subunits around the channel pore suggest that more than one arrangement may be viable.

Additional Information

© 1996 by The American Society for Biochemistry and Molecular Biology, Inc. Received June 5, 1996. Revision received July 23, 1996. We thank Paulo Kofuji for many helpful discussions. This work was supported by National Institutes of Health Grants MH49176, GM29836, NS11756, and NS34407. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

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Additional details

Identifiers

Eprint ID
29176
Resolver ID
CaltechAUTHORS:20120207-144820693

Funding

NIH
MH49176
NIH
GM29836
NIH
NS11756
NIH
NS34407

Dates

Created
2012-03-21
Created from EPrint's datestamp field
Updated
2021-11-09
Created from EPrint's last_modified field