CaltechAUTHORS
Published January 26, 2016 | Version Supplemental Material + Accepted Version
Journal Article Open

Dye-Sensitized Core/Active Shell Upconversion Nanoparticles for Optogenetics and Bioimaging Applications

Creators

  • 1. ROR icon University of Massachusetts Medical School
  • 2. ROR icon Zhejiang University
  • 3. ROR icon Pacific Northwest National Laboratory
  • 4. ROR icon University of Massachusetts Amherst
  • 5. ROR icon Lawrence Berkeley National Laboratory

Abstract

Near-infrared (NIR) dye-sensitized upconversion nanoparticles (UCNPs) can broaden the absorption range and boost upconversion efficiency of UCNPs. Here, we achieved significantly enhanced upconversion luminescence in dye-sensitized core/active shell UCNPs via the doping of ytterbium ions (Yb^(3+)) in the UCNP shell, which bridged the energy transfer from the dye to the UCNP core. As a result, we synergized the two most practical upconversion booster effectors (dye-sensitizing and core/shell enhancement) to amplify upconversion efficiency. We demonstrated two biomedical applications using these UCNPs. By using dye-sensitized core/active shell UCNP embedded poly(methyl methacrylate) polymer implantable systems, we successfully shifted the optogenetic neuron excitation window to a biocompatible and deep tissue penetrable 800 nm wavelength. Furthermore, UCNPs were water-solubilized with Pluronic F127 with high upconversion efficiency and can be imaged in a mouse model.

Additional Information

© 2016 American Chemical Society. Received: October 10, 2015; Accepted: January 6, 2016; Published: January 6, 2016. This research was supported by the China Scholarship Council (CSC) to X.W., a Worcester Foundation Mel Cutler Award, National Institutes of Health R01MH103133 to G.H, C.L., and Y.X., Human Frontier Science Program RGY-0090/2014 to G.H. and Y.X. Quantum yield work (E.M.C.) at the Molecular Foundry was supported by the U.S. Department of Energy under Contract No. DE-AC02-05CH11231. D.L. is supported by the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences Early Career Research Program, under Award No. 67037. We also thank Dr. Shaul Aloni's help in TEM measurements. Author Contributions: X. Wu, Y. Zhang, and K. Takle contributed equally to this work. The authors declare no competing financial interest.

Attached Files

Accepted Version - nihms794148.pdf

Supplemental Material - nn5b06383_si_001.pdf

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Additional details

Identifiers

PMCID
PMC4913696
Eprint ID
89976
Resolver ID
CaltechAUTHORS:20180926-144824986

Funding

China Scholarship Council
Worcester Foundation
NIH
R01MH103133
Human Frontier Science Program
RGY-0090/2014
Department of Energy (DOE)
DE-AC02-05CH11231
Department of Energy (DOE)
67037

Dates

Created
2018-09-26
Created from EPrint's datestamp field
Updated
2021-11-16
Created from EPrint's last_modified field