High-throughput force measurement of individual kinesin-1 motors during multi-motor transport
Creators
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1.
University of Illinois Urbana-Champaign
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2.
University of Vermont
Abstract
Molecular motors often work in teams to move a cellular cargo. Yet measuring the forces exerted by each motor is challenging. Using a sensor made with denatured ssDNA and multi-color fluorescence, we measured picoNewtons of forces and nanometer distances exerted by individual constrained kinesin-1 motors acting together while driving a common microtubule in vitro. We find that kinesins primarily exerted less than 1 pN force, even while the microtubule is bypassing artificial obstacles of 20–100 nanometer size. Occasionally, individual forces increase upon encountering obstacles, although at other times they do not, with the cargo continuing in a directional manner. Our high-throughput technique, which can measure forces by many motors simultaneously, is expected to be useful for many different types of molecular motors.
Additional Information
This work was supported by the NIH grants R01 GM132392 (to P. R. S.), GM120353 (to Y. R. C.), R35 GM136288 (to K. M. T.) and by the NSF Physics Frontiers Center (PFC) grant PHY-1430124 ("Center for the Physics of Living Cells").Additional details
Identifiers
- Eprint ID
- 116698
- Resolver ID
- CaltechAUTHORS:20220901-157280800
Related works
- Describes
- 10.1039/D2NR01701F (DOI)
Funding
- NIH
- R01 GM132392
- NIH
- GM120353
- NIH
- R35 GM136288
- NSF
- PHY-1430124
Dates
- Created
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2022-09-09Created from EPrint's datestamp field
- Updated
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2022-09-09Created from EPrint's last_modified field