CaltechAUTHORS
Published September 19, 2011 | Version Accepted Version
Journal Article Open

A BODIPY-Cyclooctyne for Protein Imaging in Live Cells

Creators

  • 1. ROR icon California Institute of Technology

Abstract

Cellular proteins that bear reactive azides can be imaged by fluorescence microscopy following strain-promoted ligation to cyclooctyne dyes. Here we describe BODIPY-cyclooctyne (BDPY), a membrane-permeant fluorophore that can be used to label intracellular proteins in live mammalian cells. Flow cytometry reveals fluorescence signals more than 25-fold above background after labeling of azide-tagged cells with BDPY.

Additional Information

© 2011 Wiley-VCH Verlag. Received: May 1, 2011. Published online on August 9, 2011. We thank Julie Liu, Matthew J. Hangauer, Jeremy M. Baskin, Michael S. Boyce, Andrew Mehle, and Carolyn R. Bertozzi for valuable discussions. We are grateful to Mandy K. Vink for preparing Aha and to Stacy A. Maskarinec for providing Rat-1 fibroblasts. Marissa L. Mock, Ying Ying Lu, and Rebecca E. Connor made helpful comments on the manuscript. Imaging was done at the Biological Imaging Center (Beckman Institute at Caltech). Flow cytometry was done at Caltech under the guidance of Diana Perez and Rochelle Diamond. Support was provided by a Fannie and John Hertz Foundation fellowship to K.E.B. , by an NIH postdoctoral fellowship (F32M071214) to J.D.F., and by grants from the NIH (R01M062523) and the Army Research Office (W911NF0810227).

Attached Files

Accepted Version - nihms381687.pdf

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Additional details

Identifiers

PMCID
PMC3387918
Eprint ID
27354
Resolver ID
CaltechAUTHORS:20111021-134710184

Funding

Fannie and John Hertz Foundation
NIH Postdoctoral Fellowship
F32M071214
NIH
R01M062523
Army Research Office (ARO)
W911NF0810227

Dates

Created
2011-10-21
Created from EPrint's datestamp field
Updated
2021-11-09
Created from EPrint's last_modified field