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Published January 1982 | Version Published
Journal Article Open

Amino-Terminal Sequence of the Tn3 Transposase Protein

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Abstract

The amino-terminal sequence of the Tn transposase protein was determined to be Pro-Val-Asp-Phe-Leu-Thr-Thr-Glu-Gln-Val-Glu-Ser.... This was determined both from an active transposase protein purified from a transposase overproducing mutant strain and from a hybrid transposase-β-galactosidase fusion protein. The amino acid sequence corresponded to the DNA sequence of the transposase gene beginning at an ATG initiation codon, as previously predicted from the analysis of transposase-β-galactosidase gene fusions.

Additional Information

© 1982 American Society for Microbiology. Received 20 July 1981; Accepted 16 September 1981. We thank Nicholas Cozzarelli for advice. This work was supported by Public Health Service grants GM 29067 and GM 06965 from the National Institute of General Medical Sciences to M.C. and L.H., respectively, and by National Science Foundation grant PCM 80-05599 to L.H. M.F. was supported by a fellowship from the Damon Runyon-Walter Winchell Cancer Fund (DRG-260-FT).

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Additional details

Identifiers

PMCID
PMC216644
Eprint ID
32484
Resolver ID
CaltechAUTHORS:20120716-143303838

Funding

NIH
GM 29067
NIH
GM 06965
NSF
PCM 80-05599
Damon Runyon-Walter Winchell Cancer Fund
DRG-260-FT

Dates

Created
2012-07-16
Created from EPrint's datestamp field
Updated
2019-10-03
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